Duration of the project
01.06.2023. - 15.11.2023.
Countries and institutions involved in the project
Project manager
Dr. Giedrius Steponaitis
Aims of the project
Determination of changes in the RNA-m6A epitranscriptome profile of GBM cells after co-cultivation with CIK cells.
Main activities of the project
CIK cells will be expanded in vitro from peripheral blood mononuclear cells using established protocol (UKB, Bonn, Germany)
CIK cells will be co-culture with glioblastoma cell lines with defined genetic background (UKB, Bonn, Germany)
RNA preparation: total RNA purification form cell pellets; poly-A tailed RNA enrichment (LUHS, Kaunas Lithuania)
Native RNA sequencing applying Oxford Nanopore sequencing technology (LUHS, Kaunas Lithuania)
Data analysis (LUHS, Kaunas Lithuania; UKB, Bonn, Germany)
Public events in the frame of the project
A Seminar-type lecture for PhD students and postdocs at the University Hospital Bonn by researchers from Lithuania, LUHS. Date: 20-Sep-2023
A Seminar-type lecture for PhD students and researchers at Lithuanian University of Health Sciences by researchers from Germany, UKB. Date: 11-Oct-2023
Direct and indirect target group of the project
Direct: Researcher in the field of molecular neurooncology (expected number: 5)
Indirect: PhD students; Master and bachelor students; The personnel of International Relations and Study Centre (expected number: 10)
