Analysis of RNA m6A methylation in glioblastomas using a cytokine-induced killer (CIK) cell immunotherapy approach

Duration of the project

01.06.2023. - 15.11.2023.

Countries and institutions involved in the project

Lithuanian University of Health Sciences
University Hospital Bonn

Project manager

Dr. Giedrius Steponaitis

Aims of the project

Determination of changes in the RNA-m6A epitranscriptome profile of GBM cells after co-cultivation with CIK cells.

Main activities of the project

CIK cells will be expanded in vitro from peripheral blood mononuclear cells using established protocol (UKB, Bonn, Germany)

CIK cells will be co-culture with glioblastoma cell lines with defined genetic background (UKB, Bonn, Germany)

RNA preparation: total RNA purification form cell pellets; poly-A tailed RNA enrichment (LUHS, Kaunas Lithuania)

Native RNA sequencing applying Oxford Nanopore sequencing technology (LUHS, Kaunas Lithuania)

Data analysis (LUHS, Kaunas Lithuania; UKB, Bonn, Germany)

Public events in the frame of the project

A Seminar-type lecture for PhD students and postdocs at the University Hospital Bonn by researchers from Lithuania, LUHS. Date: 20-Sep-2023

A Seminar-type lecture for PhD students and researchers at Lithuanian University of Health Sciences by researchers from Germany, UKB. Date: 11-Oct-2023

Direct and indirect target group of the project

Direct: Researcher in the field of molecular neurooncology (expected number: 5)

Indirect: PhD students; Master and bachelor students; The personnel of International Relations and Study Centre (expected number: 10)